<description>&lt;p&gt;In this podcast episode, I talk with &lt;a target="_blank" href="https://www.linkedin.com/in/francesca-giannese-08490452/"&gt;&lt;strong&gt;Francesca Giannese&lt;/strong&gt;&lt;/a&gt; about an exciting new method for studying chromatin at the single-cell level called &lt;a target="_blank" href="https://pubmed.ncbi.nlm.nih.gov/34635836/"&gt;&lt;strong&gt;scGET-seq&lt;/strong&gt;&lt;/a&gt;&lt;strong&gt;.&lt;/strong&gt; Unlike previous methods such as scATAC-seq, which only allow us to study open chromatin, &lt;strong&gt;scGET-seq&lt;/strong&gt; provides insights into both open and closed chromatin. This breakthrough can lead to a deeper understanding of how chromatin structure impacts gene regulation in individual cells. Tune in to learn more about how &lt;strong&gt;scGET-seq&lt;/strong&gt; works and its potential applications in research.&lt;/p&gt;&lt;p&gt;Contacts for support on scGET-seq implementation:&lt;/p&gt;&lt;p&gt;* &lt;a target="_blank" href="https://x.com/FrancescaGi82"&gt;&lt;strong&gt;Francesca Giannese&lt;/strong&gt;&lt;/a&gt; (wet-lab protocol implementation - enzyme requests)&lt;/p&gt;&lt;p&gt;* &lt;a target="_blank" href="https://x.com/daweonline"&gt;&lt;strong&gt;Davide Cittaro&lt;/strong&gt;&lt;/a&gt;&lt;a target="_blank" href="https://x.com/daweonline"&gt; &lt;/a&gt;(data analysis)&lt;/p&gt;&lt;p&gt;More information about scGET-seq&lt;/p&gt;&lt;p&gt;* &lt;a target="_blank" href="https://en.wikipedia.org/wiki/ScGET-seq"&gt;scGET-seq Wikipedia&lt;/a&gt; &lt;/p&gt;&lt;p&gt;* &lt;a target="_blank" href="https://www.nature.com/articles/s41587-021-01031-1"&gt;Original Paper&lt;/a&gt;&lt;/p&gt;&lt;p&gt;Data Analysis&lt;/p&gt;&lt;p&gt;* &lt;a target="_blank" href="https://star-protocols.cell.com/protocols/2563"&gt;Analyzing genomic and epigenetic profiles in single cells by hybrid transposase (scGET-seq)&lt;/a&gt;&lt;/p&gt;&lt;p&gt;* &lt;a target="_blank" href="https://www.biorxiv.org/content/10.1101/2022.06.29.498092v1"&gt;Analysis and modeling of scGET-seq data&lt;/a&gt; &lt;/p&gt;&lt;p&gt;Application&lt;/p&gt;&lt;p&gt;* &lt;a target="_blank" href="https://www.biorxiv.org/content/10.1101/2023.09.18.558231v1"&gt;Cancer evolution (EMT in pancreatic cancer)&lt;/a&gt;&lt;/p&gt;&lt;p&gt;&lt;/p&gt; &lt;br/&gt;&lt;br/&gt;This is a public episode. If you would like to discuss this with other subscribers or get access to bonus episodes, visit &lt;a href="https://thesinglecellworld.substack.com?utm_medium=podcast&amp;#38;utm_campaign=CTA_1"&gt;thesinglecellworld.substack.com&lt;/a&gt;</description>

The Single-Cell World Podcast

Catia Moutinho

Ep. 56: scGET-seq. Studying chromatin at higher resolution

OCT 17, 202438 MIN
The Single-Cell World Podcast

Ep. 56: scGET-seq. Studying chromatin at higher resolution

OCT 17, 202438 MIN

Description

<p>In this podcast episode, I talk with <a target="_blank" href="https://www.linkedin.com/in/francesca-giannese-08490452/"><strong>Francesca Giannese</strong></a> about an exciting new method for studying chromatin at the single-cell level called <a target="_blank" href="https://pubmed.ncbi.nlm.nih.gov/34635836/"><strong>scGET-seq</strong></a><strong>.</strong> Unlike previous methods such as scATAC-seq, which only allow us to study open chromatin, <strong>scGET-seq</strong> provides insights into both open and closed chromatin. This breakthrough can lead to a deeper understanding of how chromatin structure impacts gene regulation in individual cells. Tune in to learn more about how <strong>scGET-seq</strong> works and its potential applications in research.</p><p>Contacts for support on scGET-seq implementation:</p><p>* <a target="_blank" href="https://x.com/FrancescaGi82"><strong>Francesca Giannese</strong></a> (wet-lab protocol implementation - enzyme requests)</p><p>* <a target="_blank" href="https://x.com/daweonline"><strong>Davide Cittaro</strong></a><a target="_blank" href="https://x.com/daweonline"> </a>(data analysis)</p><p>More information about scGET-seq</p><p>* <a target="_blank" href="https://en.wikipedia.org/wiki/ScGET-seq">scGET-seq Wikipedia</a> </p><p>* <a target="_blank" href="https://www.nature.com/articles/s41587-021-01031-1">Original Paper</a></p><p>Data Analysis</p><p>* <a target="_blank" href="https://star-protocols.cell.com/protocols/2563">Analyzing genomic and epigenetic profiles in single cells by hybrid transposase (scGET-seq)</a></p><p>* <a target="_blank" href="https://www.biorxiv.org/content/10.1101/2022.06.29.498092v1">Analysis and modeling of scGET-seq data</a> </p><p>Application</p><p>* <a target="_blank" href="https://www.biorxiv.org/content/10.1101/2023.09.18.558231v1">Cancer evolution (EMT in pancreatic cancer)</a></p><p></p> <br/><br/>This is a public episode. If you would like to discuss this with other subscribers or get access to bonus episodes, visit <a href="https://thesinglecellworld.substack.com?utm_medium=podcast&#38;utm_campaign=CTA_1">thesinglecellworld.substack.com</a>