<p>This episode of MCAT Basics covers several separation and purification techniques you may see on the MCAT. We’ll cover these techniques in three distinct categories: small molecules, proteins, and cells. </p> <p>Within the small molecule category, we’ll discuss extraction, crystallization and recrystallization, and chromatography (TLC and HPLC).</p> <p>For proteins, we cover SDS-page, precipitation, soelectric focusing, and chromatography variations. Lastly, we’ll explain, within the world of cells, centrifugation, flow cytometry, and microfluidics. </p> <p><strong> </strong></p> <p>Visit <a href="https://www.medschoolcoach.com/mcat-tutoring" rel="ugc noopener noreferrer" target="_blank">MedSchoolCoach.com</a> for more help with the MCAT. </p> <p><strong> </strong></p> <p>Jump into the conversation:</p> <p>[00:00] MCAT Tutorig from MedSchoolCoach</p> <p>[00:34] Welcome to MCAT Basics</p> <p>[01:07] Topics covered in this episode</p> <p>[02:24] Techniques for small molecules - extraction, crystallization and recrystallization, and chromatography (TLC and HPLC)</p> <p>[28:24] Techniques for proteins - SDS-page, precipitation, isoelectric focusing, and chromatography variations</p> <p>[54:47] Techniques for cells - centrifugation, flow cytometry, and microfluidics. </p> <p> </p>
![<p>This episode of MCAT Basics covers several separation and purification techniques you may see on the MCAT. We’ll cover these techniques in three distinct categories: small molecules, proteins, and cells. </p> <p>Within the small molecule category, we’ll discuss extraction, crystallization and recrystallization, and chromatography (TLC and HPLC).</p> <p>For proteins, we cover SDS-page, precipitation, soelectric focusing, and chromatography variations. Lastly, we’ll explain, within the world of cells, centrifugation, flow cytometry, and microfluidics. </p> <p><strong> </strong></p> <p>Visit <a href="https://www.medschoolcoach.com/mcat-tutoring" rel="ugc noopener noreferrer" target="_blank">MedSchoolCoach.com</a> for more help with the MCAT. </p> <p><strong> </strong></p> <p>Jump into the conversation:</p> <p>[00:00] MCAT Tutorig from MedSchoolCoach</p> <p>[00:34] Welcome to MCAT Basics</p> <p>[01:07] Topics covered in this episode</p> <p>[02:24] Techniques for small molecules - extraction, crystallization and recrystallization, and chromatography (TLC and HPLC)</p> <p>[28:24] Techniques for proteins - SDS-page, precipitation, isoelectric focusing, and chromatography variations</p> <p>[54:47] Techniques for cells - centrifugation, flow cytometry, and microfluidics. </p> <p> </p>](https://us-central1-podcastguru-217d6.cloudfunctions.net/gcf_get_image?image_url=https://is1-ssl.mzstatic.com/image/thumb/Podcasts211/v4/cc/f8/5e/ccf85e68-a9fd-0e2a-4744-f90a8de3f636/mza_10513669359961335562.png/600x600bb.jpg&width=300&height=300&api_key=xqFmUFX5BHLBTA)
